Part:BBa_K257011:Design

PBad + clyA_CTerm + RFP_NTerm

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1302
  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  Illegal AgeI site found at 2712
  Illegal AgeI site found at 2824
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  

Design Notes

The fusion protein is designed following the standard 23 in order to avoid frameshift in the sequence.

Source

It's a composite part. PBad comes from the registry (2008 distribution). ClyA_CTerm :: RFP_NTerm is the BBa_K257010.